Cell-Free Protein Synthesis: A Breakthrough in Modern Biotechnology
Cell-free protein synthesis (CFPS) is emerging as one of the most innovative techniques in biotechnology, offering a flexible and efficient approach to producing proteins without relying on living cells. Unlike traditional methods that depend on culturing bacteria, yeast, or mammalian cells, CFPS operates in a controlled environment outside of the cell, utilizing the cellular machinery extracted from organisms to drive protein production. This revolutionary approach is transforming research, drug development, and industrial biotechnology.
At its core, CFPS harnesses the transcription and translation machinery that normally functions inside living cells. Key components such as ribosomes, enzymes, amino acids, and nucleotides are combined in a reaction mixture, enabling rapid protein synthesis in a test tube or bioreactor. Because the system bypasses the need to grow and maintain living cells, it significantly reduces the time required to produce proteins, allowing researchers to generate results within…



